Composite

Part:BBa_K571010:Design

Designed by: Lu-Chu Ke   Group: iGEM11_TzuChiU_Formosa   (2011-10-11)


R0011+acsCD+CMCax+Ccp/pSB1C3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3233
    Illegal EcoRI site found at 6371
    Illegal PstI site found at 143
    Illegal PstI site found at 329
    Illegal PstI site found at 779
    Illegal PstI site found at 2615
    Illegal PstI site found at 2945
    Illegal PstI site found at 3071
    Illegal PstI site found at 4144
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3233
    Illegal EcoRI site found at 6371
    Illegal PstI site found at 143
    Illegal PstI site found at 329
    Illegal PstI site found at 779
    Illegal PstI site found at 2615
    Illegal PstI site found at 2945
    Illegal PstI site found at 3071
    Illegal PstI site found at 4144
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3233
    Illegal EcoRI site found at 6371
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3233
    Illegal EcoRI site found at 6371
    Illegal PstI site found at 143
    Illegal PstI site found at 329
    Illegal PstI site found at 779
    Illegal PstI site found at 2615
    Illegal PstI site found at 2945
    Illegal PstI site found at 3071
    Illegal PstI site found at 4144
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3233
    Illegal EcoRI site found at 6371
    Illegal PstI site found at 143
    Illegal PstI site found at 329
    Illegal PstI site found at 779
    Illegal PstI site found at 2615
    Illegal PstI site found at 2945
    Illegal PstI site found at 3071
    Illegal PstI site found at 4144
    Illegal NgoMIV site found at 662
    Illegal NgoMIV site found at 1738
    Illegal NgoMIV site found at 2858
    Illegal NgoMIV site found at 5083
    Illegal NgoMIV site found at 5834
    Illegal AgeI site found at 874
    Illegal AgeI site found at 2165
    Illegal AgeI site found at 4501
    Illegal AgeI site found at 5140
    Illegal AgeI site found at 5993
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It is a very long sequence, therefore below is a few measures to take note during cloning.

  • The concentration agarose gel during gel electrophoresis should be about 0.8-1%
  • The enzyme digestion reaction time is advised to be elongated so that digestion could be more perfect.
  • The respective restriction enzyme at the both end of primer should not cut the sequence of another gene.


Source

  • promoter: iGEM registry of standard biological part
  • acsCD: GenBank AB091060.1
  • CMCax: GenBank EFG85213.1
  • Ccp: GenBank AAA16970.1

References